Gambaran Histologis Sel Piramidal Hipokampus Tikus Putih (Rattus Norvegicus) Jantan Dewasa Strain Wistar Pasca Penghentian Pajanan Monosodium Glutamat Peroral

Dayono, Buddy (2015) Gambaran Histologis Sel Piramidal Hipokampus Tikus Putih (Rattus Norvegicus) Jantan Dewasa Strain Wistar Pasca Penghentian Pajanan Monosodium Glutamat Peroral. Skripsi thesis, Universitas Tanjungpura.

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Abstract

Abstrak Latar Belakang Monosodium glutamat (MSG) merupakan salah satu bahan penyedap masakan yang berperan menciptakan rasa umami. Penggunaan MSG melebihi ambang batas menyebabkan kerusakan hipokampus. Tujuan: penelitian ini bertujuan untuk mengetahui gambaran histologis sel piramidal hipokampus tikus putih (Rattus norvegicus) jantan dewasa strain wistar pasca penghentian pajanan MSG peroral. Metodologi: Desain penelitian ini merupakan true eksperimental. Penelitian ini menggunakan 27 tikus dan dibagi 9 kelompok. Sampel dipilih dengan metode simple random sampling. Kelompok kontrol positif (KP) diberikan akuadest 1,5ml selama 28, 42, 56 hari; Kelompok kontrol negatif (KN) diberikan MSG 5mg/gBB/hari selama 28, 42, 56 hari; Kelompok perlakuan (P) diberikan MSG 5mg/gBB/hari selama 28 hari dan dihentikan selama 1, 14, 28 hari. Tikus kemudian dibedah secara bertahap pada hari ke-29, ke-43 dan ke-57. Hipokampus dibuat preparat mikroskopik dengan pewarnaan H&E. Variabel data adalah jumlah sel piramidal normal dan rusak. Data diamati pada kedua hipokampus dengan perbesaran lensa objektif 40x. Data dianalisa menggunakan one-way anova dilanjutkan LSD dan Kruskal Wallis Test dilanjutkan Mann-Whitney Test. Analisa data menggunakan program SPSS versi 16.0. Hasil: Tidak terdapat perbedaan bermakna rerata jumlah sel piramidal rusak pada seluruh kelompok perlakuan (P) pada hari yang berbeda (p≥0,05). Terdapat perbedaan bermakna rerata jumlah sel piramidal normal pada seluruh kelompok perlakuan (P) pada hari yang berbeda (p<0,05). Kesimpulan: Penggunaan MSG melebihi ambang batas menyebabkan kerusakan hipokampus berupa degenerasi sel piramidal dan terjadi regenerasi pada 28 hari pasca penghentian pajanan MSG. Abstract Background: Monosodium glutamate (MSG) is a flavor enhancher which used for arising umami taste. Excessive consumption of MSG resulted in hippocampal pyramidal cells degeneration. Objective: The aim of this study was to determine the histological of hippocampal pyramidal cells adult male wistar rats (Rattus norvegicus) after cessation of oral administration monosodium glutamate. Methodology: This is true experimental study. This study used 27 rats and divided into 9 groups with simple random sampling. Positive control groups (KP) were given aquadest 1,5ml for 28, 42, 56 days; Negative control groups (KN) were given MSG dose 5mg/gBW/day for 28, 42, 56 days; Treatment groups (P) were given MSG dose 5mg/gBW/day for 28 days and stopped for 1, 14, 28 days .The rats were then dissected gradually at day-29, day-43 and days-57. The hippocampus was processes into microscopic preparations and stained with H&E. Measured variables include normal and abnormal pyramidal cells. The variables data were observed in the both side of hippocampus with a magnification of 40x objective lens. Data were analyzed using One-way ANOVA followed by LSD and Kruskal Wallis Test followed by Mann-Whitney Test. Data analysis using SPSS version 16.0 Results: There were no significant differences in the mean number of pyramidal cells degeneration between treatment groups (P) (p≥0,05). There were significant differences in the mean number of normal pyramidal cells between treatment groups (P) (p<0,05). Conclusions: Excessive consumption of MSG resulted in hippocampal pyramidal cells degeneration and regeneration occur in 28 days after withdrawal of exposure to MSG for 28 days.

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